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The spectroscopic ruler is a noninvasive technique exploiting fluorescence resonance energy transfer (FRET) to determine intramolecular distances on the nm scale in proteins and other bio-molecules [1,2]. It is widely applied in the life sciences where it is used in vitro and in vivo to probe the separation between fluorescent markers attached to the molecules under investigation. Because FRET scales strongly with the separation distance and orientation between the optical dipole moments of the donor and acceptor molecules, the accuracy of the technique is limited when the molecules undergoes thermal fluctuations under physiological conditions and the orientation of the optical dipoles is not static. © 2011 IEEE.

Original publication

DOI

10.1109/CLEOE.2011.5943241

Type

Journal article

Journal

2011 Conference on Lasers and Electro-Optics Europe and 12th European Quantum Electronics Conference, CLEO EUROPE/EQEC 2011

Publication Date

06/09/2011