Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In this paper we describe the first example of the use of an electroactive label as a SERS marker for detecting and quantifying target oligonucleotides and distinguishing mutations using a combination of voltammetry and electrochemically induced melting (E-melting). The experiments were carried out on sphere segment void substrates selected to show strong surface enhancement for SERS at 633 nm. DNA analysis was carried out for CFTR sequences using synthetic 22-mer oligonucleotides labelled with a modified anthraquinone at the 3'-end. Discrimination between the wild type, 1653C/T (point mutation) and DF508 (triple deletion) is demonstrated. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original publication

DOI

10.1002/elan.200904681

Type

Journal article

Journal

Electroanalysis

Publication Date

01/10/2009

Volume

21

Pages

2190 - 2197