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The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA.

Original publication

DOI

10.1002/0471142700.nc0433s35

Type

Journal article

Journal

Curr Protoc Nucleic Acid Chem

Publication Date

12/2008

Volume

Chapter 4

Pages

Unit - 4.33

Keywords

Alkynes, Azides, Catalysis, Copper, Cyclization, DNA, Nucleic Acid Conformation, Oligonucleotides