Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR SGM Plus system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1h.

Original publication

DOI

10.1016/j.fsigen.2008.04.007

Type

Journal article

Journal

Forensic Sci Int Genet

Publication Date

09/2008

Volume

2

Pages

333 - 339

Keywords

DNA, DNA Fingerprinting, Databases, Nucleic Acid, Fluorescent Dyes, Genetic Testing, Humans, Microsatellite Repeats, Mouth Mucosa, Nucleic Acid Denaturation, Nucleic Acid Hybridization, Pedigree, Polymerase Chain Reaction