Synthesis of a modified thymidine monomer for site-specific incorporation of reporter groups into oligonucleotides
Brown LJ., May JP., Brown T.
The efficient incorporation of reporter groups into oligonucleotides at specific sites has been facilitated by the synthesis of a novel modified thymidine monomer with an FMOC-protected hydroxyl group on a linker. The primary hydroxyl group can be deprotected during or after solid-phase oligonucleotide synthesis and reacted with any reporter phosphoramidite. © 2001 Elsevier Science Ltd.