Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

2',4'-Dideoxy-4'-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4'-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4'-oxygen in recognition and cleavage of dU residues in DNA.

Original publication

DOI

10.1080/15257770008045442

Type

Journal article

Journal

Nucleosides Nucleotides Nucleic Acids

Publication Date

10/2000

Volume

19

Pages

1505 - 1516

Keywords

Base Sequence, DNA Glycosylases, DNA Repair, Herpesvirus 1, Human, Magnetic Resonance Spectroscopy, N-Glycosyl Hydrolases, Nucleotides, Surface Plasmon Resonance, Uracil-DNA Glycosidase