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We report the development of a novel detection and typing methodology for human papillomaviruses (HPV) based on real-time PCR with the self-probing fluorescent primers known as Scorpions. This technique is quick, simple, specific, sensitive, and capable of estimating viral load per cell. We report the results of over 100 typing reactions performed on cell lines, biopsies, and cervical cytobrush samples which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. We further report preliminary data suggesting a relationship between viral load per cell and grade of cervical disease.

Original publication

DOI

10.1128/jcm.39.9.3204-3212.2001

Type

Journal article

Journal

J Clin Microbiol

Publication Date

09/2001

Volume

39

Pages

3204 - 3212

Keywords

Cervix Uteri, DNA Primers, DNA, Viral, Female, Humans, Immunoenzyme Techniques, Papillomaviridae, Papillomavirus Infections, Polymerase Chain Reaction, Tumor Cells, Cultured, Tumor Virus Infections, Viral Load