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Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.

Original publication

DOI

10.1039/c3cc45917a

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

18/01/2014

Volume

50

Pages

551 - 553

Keywords

5-Methylcytosine, CpG Islands, DNA, DNA Methylation, Hydrogen-Ion Concentration, Nucleic Acid Hybridization, Oligonucleotides, Transition Temperature