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A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3'-azide and 5'-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.

Original publication

DOI

10.1039/c4cc03027c

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

21/07/2014

Volume

50

Pages

7597 - 7600

Keywords

Genetic Linkage, MicroRNAs, Reverse Transcription, Sequence Analysis, RNA, Triazoles