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The amino group of 5-aminopropargyl-2'-deoxyuridine-5'-triphosphate was labelled with dibenzocyclooctyne (DIBO) and two derivatives of bicyclo [6.1.0] non-4-yne (BCN) with short and long linkers to produce three different cycloalkyne-modified deoxyuridine triphosphates. BCN was successfully incorporated into DNA at multiple sites by enzyme-mediated primer extension and the polymerase chain reaction (PCR). Efficient fluorescent labelling of the BCN-DNA and DIBO-DNA with Cy3-azide was demonstrated.

Original publication

DOI

10.1016/j.bmc.2014.05.050

Type

Journal article

Journal

Bioorg Med Chem

Publication Date

15/08/2014

Volume

22

Pages

4384 - 4390

Keywords

BCN, Copper free click, Cyclooctyne deoxyuridine triphosphates, DIBO, DNA fluorescent labelling, Cyclooctanes, DNA, DNA-Directed DNA Polymerase, Deoxyuracil Nucleotides, Fluorescence, Fluorescent Dyes