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The amino group of 5-aminopropargyl-2′-deoxyuridine-5′- triphosphate was labelled with dibenzocyclooctyne (DIBO) and two derivatives of bicyclo [6.1.0] non-4-yne (BCN) with short and long linkers to produce three different cycloalkyne-modified deoxyuridine triphosphates. BCN was successfully incorporated into DNA at multiple sites by enzyme-mediated primer extension and the polymerase chain reaction (PCR). Efficient fluorescent labelling of the BCN-DNA and DIBO-DNA with Cy3-azide was demonstrated. © 2014 Published by Elsevier Ltd. All rights reserved.

Original publication




Journal article


Bioorganic and Medicinal Chemistry

Publication Date





4384 - 4390