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Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL-A typing showed the expected HL-A antigens plus a considerable number of additional reactions. Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated years later.

Type

Journal article

Journal

Tissue Antigens

Publication Date

03/1976

Volume

7

Pages

165 - 172

Keywords

Blood Preservation, Cell Line, Cell Separation, Cell Survival, Chromosomes, Consanguinity, Epitopes, Freezing, HLA Antigens, Histocompatibility Antigens, Histocompatibility Testing, Humans, Lymphocyte Activation, Lymphocytes, Time Factors