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We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

Original publication

DOI

10.1021/ja512416n

Type

Journal article

Journal

J Am Chem Soc

Publication Date

25/02/2015

Volume

137

Pages

2444 - 2447

Keywords

Base Sequence, DNA, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Models, Molecular, Nucleic Acid Conformation