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Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.

Original publication




Journal article


J Am Chem Soc

Publication Date





9069 - 9072


Alkynes, Base Sequence, DNA, B-Form, Electron Spin Resonance Spectroscopy, Models, Molecular, Nucleic Acid Conformation, Nucleotides, Spin Labels