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Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3-Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.

Original publication

DOI

10.1002/cbic.201700286

Type

Journal article

Journal

Chembiochem

Publication Date

19/09/2017

Volume

18

Pages

1814 - 1818

Keywords

FRET, click chemistry, fluorescent probes, in organello synthesis, mitochondrial targeting, Animals, Carbocyanines, Cell Line, Click Chemistry, Copper, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Microscopy, Confocal, Mitochondria, Rats