Investigating d-lysine stereochemistry for epigenetic methylation, demethylation and recognition.
Belle R., Al Temimi AHK., Kumar K., Pieters BJGE., Tumber A., Dunford JE., Johansson C., Oppermann U., Brown T., Schofield CJ., Hopkinson RJ., Paton RS., Kawamura A., Mecinović J.
Histone lysine methylation is regulated by Nε-methyltransferases, demethylases, and Nε-methyl lysine binding proteins. Thermodynamic, catalytic and computational studies were carried out to investigate the interaction of three epigenetic protein classes with synthetic histone substrates containing l- and d-lysine residues. The results reveal that out of the three classes, Nε-methyl lysine binding proteins are superior in accepting lysines with the d-configuration.