Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries.
Matysiak S., Hellmuth K., El-Sagheer AH., Shivalingam A., Ariyurek Y., de Jong M., Hollestelle MJ., Out R., Brown T.
DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design.