Sheikh S., Coutts AS., La Thangue NB.
© 2017 Elsevier Inc. All rights reserved. Over the last 30 years, the transfection of DNA or RNA into mammalian cells has evolved into an ever more sophisticated research tool for the investigation of gene expression and protein function. Transfection is used to describe the process of introducing foreign nucleic acid into a cell. Here we aim to give an overview of commonly used transfection methods, their application, and relative advantages and disadvantages. We will then present a current transfection protocol as a worked example and highlight practical issues such as how to troubleshoot and optimize an existing transfection protocol.