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Seven phage clones containing human sequences were picked at random from a human genomic library cloned in Charon 4A. The clones are devoid of repetitive sequences and can be used to recognize restriction fragment length polymorphisms (Feder et al. 1985). The chromosomal locations of the sequences defined by the seven clones have been determined by Southern blotting and DNA hybridization to DNA from human-mouse somatic cell hybrids. The chromosomal assignment of these sequences should increase their value as genetic markers in family studies.

Type

Journal article

Journal

Ann Hum Genet

Publication Date

05/1986

Volume

50

Pages

145 - 152

Keywords

Animals, Autoradiography, Bacteriophage lambda, Base Sequence, Chromosomes, Human, Cloning, Molecular, DNA, Genetic Markers, Humans, Hybrid Cells, Mice, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length