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We have studied the role of cell-matrix interactions and the modulating effect on these of transforming growth factor-beta s (TGF-beta s) in controlling differentiation and proliferation in a series of human colorectal carcinoma cell lines. Two (SW1222 and SW480) out of 7 cell lines specifically bound type-I collagen, via integrin-like (SW1222) and non-integrin-like (SW480) collagen receptors. Binding of these receptors may be responsible for regulating the degree of epithelial differentiation of the cells when grown in a 3-dimensional (3D) collagen gel. We have also shown that TGF-beta s enhance the binding of SW1222 cells to collagen and that this is accompanied by greatly increased crypt-like glandular differentiation and inhibition of cell proliferation. Inhibition of cell proliferation was only seen when cells were grown in 3D collagen gel and were thus expressing a fully differentiated phenotype. The enhanced collagen binding induced by TGF-beta s was partially inhibited by an Arg-Gly-Asp (RGD)-containing peptide which is a cell recognition signal for collagen binding. This suggests that TGF-beta s mediate their effects on differentiation of SW1222 cells specifically by modulating the expression of the integrin-like collagen receptor. The other colorectal carcinoma cell lines which lack this integrin-like receptor either failed to bind collagen or, in the case of SW480 binding, exhibited differentiation and proliferation which were not affected by TGF-beta s. This suggests that cell responsiveness to TGF-beta s may depend, at least in part, upon the cell-matrix interaction.

Type

Journal article

Journal

Int J Cancer

Publication Date

15/09/1989

Volume

44

Pages

518 - 523

Keywords

Cell Differentiation, Cell Division, Collagen, Colorectal Neoplasms, Humans, Integrins, Membrane Glycoproteins, Receptors, Cell Surface, Receptors, Collagen, Transforming Growth Factors, Tumor Cells, Cultured