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Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.

Original publication

DOI

10.1038/1394

Type

Journal article

Journal

Nat Struct Biol

Publication Date

08/1998

Volume

5

Pages

697 - 701

Keywords

Bacterial Proteins, Cisplatin, Computer Simulation, Crystallography, DNA Adducts, DNA Repair, Models, Molecular, Molecular Conformation, N-Glycosyl Hydrolases, Oligodeoxyribonucleotides, Recombinant Proteins, Thymine DNA Glycosylase