Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.

Original publication

DOI

10.1038/1394

Type

Journal article

Journal

Nat Struct Biol

Publication Date

08/1998

Volume

5

Pages

697 - 701

Keywords

Bacterial Proteins, Cisplatin, Computer Simulation, Crystallography, DNA Adducts, DNA Repair, Models, Molecular, Molecular Conformation, N-Glycosyl Hydrolases, Oligodeoxyribonucleotides, Recombinant Proteins, Thymine DNA Glycosylase