Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In this section, we review methods for the analysis of the CFTR gene and its transcript. First, we discuss techniques to accurately measure levels of CFTR mRNA in primary human cells; next, protocols for measuring CFTR transcripts that contain premature termination codons and for evaluating the role of nonsense-mediated decay which targets these transcripts; a further chapter considers methodology to investigate pre-mRNA splicing. The penultimate chapter concentrates on methods for evaluating microRNA regulation of gene expression in the context of airway disease. The final chapter considers methods to evaluate the chromatin structure of the active CFTR locus and to analyse the cis-acting regulatory elements that control it.

Original publication




Journal article


Methods Mol Biol

Publication Date





111 - 113


Cystic Fibrosis Transmembrane Conductance Regulator, Gene Expression Regulation, Genetic Techniques, Humans, RNA, Messenger