Figure 6 from VCAM-1–targeted MRI Improves Detection of the Tumor-brain Interface
Cheng VWT., de Pennington N., Zakaria R., Larkin JR., Serres S., Sarkar M., Kirkman MA., Bristow C., Croal P., Plaha P., Campo L., Chappell MA., Lord S., Jenkinson MD., Middleton MR., Sibson NR.
<p>Peritumoral vasculature is characterized by upregulated endothelial VCAM-1 expression. Representative histologic sections of peritumoral brain tissue, from breast cancer (<b>A</b>), lung adenocarcinoma (<b>B</b>), and melanoma brain metastases (<b>C</b>) immunohistochemically stained for the endothelial marker, CD34 (brown); scale bar = 200 μm, black dashed line outlines tumor border. <b>D–F,</b> Corresponding graphs showing mean microvessel density (±SD) in the peritumoral brain parenchyma in the following regions: < 0.5 mm, 0.5–1 mm, and > 1 mm from the metastasis border; <i>n</i> = 10 (breast), <i>n</i> = 9 (lung), and <i>n</i> = 12 (melanoma). <b>G–I,</b> Images showing endothelial VCAM-1 expression (arrowheads) associated with the metastasis from the same patients as above; scale bar = 100 μm. <b>J–L,</b> Corresponding graphs showing ratio of VCAM-1–positive/negative vessels in randomly distributed square ROIs across the peritumoral brain parenchyma. <b>M,</b> Representative image of endothelial VCAM-1 expression at the border of a glioblastoma, with corresponding graph of VCAM-1–positive/negative vessel distribution (<b>N</b>); scale bar = 100 μm.</p>