Figure 5 from VCAM-1–targeted MRI Improves Detection of the Tumor-brain Interface
Cheng VWT., de Pennington N., Zakaria R., Larkin JR., Serres S., Sarkar M., Kirkman MA., Bristow C., Croal P., Plaha P., Campo L., Chappell MA., Lord S., Jenkinson MD., Middleton MR., Sibson NR.
<p>Spatial colocalization of VCAM-1 and VCAM-MPIO–induced hypointensities in metastasis and medulloblastoma models. Representative images from a MDA231Br-GFP tumor-bearing animal (<b>A–F</b>) and a DAOY medulloblastoma animal (<b>G–L</b>). Positive VCAM-1 expression (brown) is evident on the endothelium of vessels at the margins of both the MDA231Br-GFP tumor (<b>A</b> and <b>D</b>) and the DAOY tumor (<b>G</b> and <b>J</b>); cresyl violet counterstain (blue). Scale bar = 1 mm. Inset images in <b>A</b> and <b>G</b> illustrate presence of VCAM-MPIO beads (black arrowhead) bound to the luminal surface of VCAM-1–positive vessels (brown); scale bar = 10 μm. <b>B</b>, <b>E</b>, <b>H</b>, and <b>K</b>, Corresponding high-powered magnification images from boxes; scale bar = 100 µm. <b>D</b>, <b>E</b>, <b>J</b>, and <b>K</b>, Areas segmented as VCAM-1 positive are highlighted in red. Note, in <b>A</b> and <b>D</b> nonspecific staining of the ventricles and tissue edges is evident, which has not been removed from the analysis for transparency, but does not reflect true endothelial VCAM-1 staining. <b>C</b>, <b>F</b>, <b>I</b>, and <b>L</b>, Corresponding <i>T</i><sub>2</sub><i>*</i>-weighted MRI slices showing VCAM-MPIO–induced hypointensities either alone (<b>C</b> and <b>I</b>), or with an overlay (red) showing perspective transformed histology derived VCAM-1 staining (<b>F</b> and <b>L</b>); scale bar = 1 mm.</p>