Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Ionising radiation can induce responses within non-exposed neighbouring (bystander) cells, which potentially have important implications on the estimates of risk at environmentally relevant doses. Using human skin fibroblasts (AG1522), a range of methods were used to investigate the nature of the signal(s) arising from the exposed cells. The signal(s) can be transmitted by direct cell-cell communication (investigated by using partial dish irradiations) or by medium-borne factors (a co-culture system where two monolayers share the same medium but only one monolayer is exposed to ionising radiation). CDKN1A was found to be up-regulated in both directly exposed and non-exposed cells. The data suggest that direct cell-cell communication dominates for these confluent cells, with medium-borne factors also contributing.

Original publication

DOI

10.1093/rpd/ncl430

Type

Journal article

Journal

Radiat Prot Dosimetry

Publication Date

2006

Volume

122

Pages

266 - 270

Keywords

Bystander Effect, Cell Line, Cyclin-Dependent Kinase Inhibitor p21, DNA, DNA Damage, Dose-Response Relationship, Radiation, Enzyme Activation, Fibroblasts, Humans, Radiation Dosage, Radiation Tolerance