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Investigation of the mechanisms behind the regulation of cellular motility and adhesion is key to understanding metastasis and the biology of tumor spreading. There are many technologies available for these studies, but the majority of them are either dependent on the use of labels or limited to endpoint analysis. The xCELLigence RTCA (real-time cell analysis) provides a platform for label free and operator independent investigation of the migration, invasion and adhesion proprieties of cells in physiologically relevant conditions. The real-time kinetic data acquisition also allows for a more accurate characterization of short-lived cellular events. In this chapter we describe the use of the xCELLigence Real-Time Cell Analyzer to investigate changes in cellular adhesion and motility in real time.

Original publication

DOI

10.1007/978-1-62703-538-5_17

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2013

Volume

1046

Pages

295 - 306

Keywords

Cell Adhesion, Cell Movement, Humans, Molecular Biology, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms