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Understanding the mechanisms of chromosomal double-strand break repair (DSBR) provides insight into genome instability, oncogenesis and genome engineering, including disease gene correction. Research into DSBR exploits rare-cutting endonucleases to cleave exogenous reporter constructs integrated into the genome. Multiple reporter constructs have been developed to detect various DSBR pathways. Here, using a single endogenous reporter gene, the X-chromosomal disease gene encoding hypoxanthine phosphoribosyltransferase (HPRT), we monitor the relative utilization of three DSBR pathways following cleavage by I-SceI or CRISPR/Cas9 nucleases. For I-SceI, our estimated frequencies of accurate or mutagenic non-homologous end-joining and gene correction by homologous recombination are 4.1, 1.5 and 0.16%, respectively. Unexpectedly, I-SceI and Cas9 induced markedly different DSBR profiles. Also, using an I-SceI-sensitive HPRT minigene, we show that gene correction is more efficient when using long double-stranded DNA than single- or double-stranded oligonucleotides. Finally, using both endogenous HPRT and exogenous reporters, we validate novel cell cycle phase-specific I-SceI derivatives for investigating cell cycle variations in DSBR. The results obtained using these novel approaches provide new insights into template design for gene correction and the relationships between multiple DSBR pathways at a single endogenous disease gene.

Original publication

DOI

10.1093/hmg/ddv409

Type

Journal article

Journal

Hum Mol Genet

Publication Date

15/12/2015

Volume

24

Pages

7097 - 7110

Keywords

Animals, Bacterial Proteins, CRISPR-Associated Protein 9, CRISPR-Cas Systems, Cell Cycle, Cell Line, Tumor, DNA Breaks, Double-Stranded, DNA Repair, Deoxyribonucleases, Type II Site-Specific, Endonucleases, Genes, Reporter, HeLa Cells, Humans, Hypoxanthine Phosphoribosyltransferase, Mice, Mutagenesis, Saccharomyces cerevisiae Proteins