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Base excision repair is the major pathway for the repair of oxidative DNA damage in human cells that is initiated by a damage-specific DNA glycosylase. In human cells, the major DNA glycosylases for the excision of oxidative base damage are OGG1 and NTH1 that excise 8-oxoguanine and oxidative pyrimidines, respectively. We find that both enzymes have limited activity on DNA lesions located in the vicinity of the 3' end of a DNA single-strand break, suggesting that other enzymes are involved in the processing of such lesions. In this study, we identify and characterize NEIL1 as a major DNA glycosylase that excises oxidative base damage located in close proximity to the 3' end of a DNA single-strand break.

Original publication

DOI

10.1093/nar/gki816

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

2005

Volume

33

Pages

4849 - 4856

Keywords

Base Sequence, DNA Damage, DNA Glycosylases, DNA Repair, Deoxyribonuclease (Pyrimidine Dimer), Guanine, Humans, Oligonucleotides, Oxidative Stress, Substrate Specificity, Uracil