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A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.

Original publication




Journal article


Proc Natl Acad Sci U S A

Publication Date





11338 - 11343


Base Sequence, DNA, DNA-Directed DNA Polymerase, Escherichia coli, Molecular Mimicry, Molecular Sequence Data, Molecular Structure, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Triazoles